FIGURE 4.

(A,B) Comparison of myelination and remyelination in live cerebellar explant cultures of Tnr ^+/+ and Tnr ^−/− mice. (A) Tnr ^+/+ and Tnr ^−/− cerebellar explant cultures of the conditions myelinated (M), demyelinated (DM), remyelinated (RM), and control (C) were immunohistochemically labeled with antibodies against NF200 (red) and MBP (green) to visualize the wrapping of myelin membranes around nerve fibers. Colocalization of neurofilament and MBP appeared as yellow staining. Demyelination nearly eliminated MBP-labeling. The myelinated condition shows a highly distinctive MBP staining. An initiating remyelination is visible for both genotypes. (B) Quantification of myelination indices of each condition in the comparison of Tnr ^+/+ and Tnr ^−/− explant cultures (M, myelinated; DM, demyelinated; RM, remyelinated; C, control). For the demyelinated condition, the quantification shows a significant reduction of the myelination index compared to the other conditions. Overall, both myelination and remyelination appeared more extensive in the absence of Tnr. All data are provided as mean ± SEM. Statistical significance was assessed using the two-way ANOVA (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001) and Tukey’s multiple-comparisons test. Scale bars: 50 µm. Nine independent experiments were performed (N = 9), and three explants (n = 3) were analyzed per experiment for each condition and each slice culture group.