FIGURE 5.

(A,B) Comparison of remyelination efficiency in cerebellar cultures from NMRI-mice in the presence of purified Tnr. (A) Labeling for neurofilament (red) and MBP (green) of live cerebellar explants kept under four different conditions: myelinated, demyelinated, remyelinated, and control. Explants were complemented either with plain PBS (control), with 10 µg, or with 20 µg immunopurified Tnr applied to the explant culture medium. MBP labeling indicates the degree of myelination under the different conditions. (B) The graph displays the quantification of myelination indices of the distinct conditions PBS-control (PBS-C), 10 µg Tnr, and 20 µg Tnr as applied to explant cultures kept in the different conditions (M, myelinated; DM, demyelinated; RM, remyelinated; C, control). All data are provided as mean ± SEM. Statistical significance was assessed using the two-way ANOVA (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001) and Tukey’s multiple-comparisons test. Scale bars: 50 µm. Five independent experiments (N = 5) were carried out, and three (n = 3) explants per experiment were analyzed for each condition and each slice culture group.