FIGURE 7.

(A–C) Comparison of Olig2/CC1-double immunolabeling in the corpus callosum of SV129, Tnc ^−/− , and Tnr ^−/− mice under control, demyelinated, and remyelinated conditions. (A) Using immunohistochemical labeling with antibodies against Olig2 (detects all oligodendroglia) and CC1 (detects mature oligodendrocytes), the percentage of mature oligodendrocytes in the corpus callosum of SV129, Tnc ^−/− , and Tnr ^−/− mice was compared. The four conditions “control,” “demyelinated,” “one-week remyelinated,” and “two-weeks remyelinated” were examined. DAPI-staining marks cell nuclei in blue color. Olig2 is nucleus-based staining, and CC1 is located in the cytosol. In the absence of treatment, a strong labeling of CC1-positive cells (green) was detectable in the corpus callosum (CC) of all genotypes. Mature CC1/Olig2-positive cells were reduced in the demyelinated condition. (B) Quantification of Olig2-positive cells in the particular conditions (C: control, DM: demyelinated, RM1: one-week remyelinated, RM2: two-weeks remyelinated). (C) Quantification of CC1/Olig2-double immunopositive cells. The quantification confirms a lower percentage of mature oligodendrocytes in the demyelinated condition compared to the control for all genotypes. All data are provided as mean ± SEM. The statistical significance was assessed by using the two-way ANOVA (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001) and Tukey’s multiple-comparisons test. The micrograph of the illustration was captured via Axiophot. Scale bars: illustration: 100 µm detail, pictures: 50 μm; at least N = 3 animals were used for each group and genotype, and at least n = 500 cells were evaluated for each individual animal.