FIGURE 2.

Expression pattern of SlNAC063. (A) Analysis of SlNAC063 expression in different tissues. Seedlings with 3–4 cm root length were transferred to the solution containing 0 or 5 μM Al³⁺ for 6 h. RNA was extracted from root tip (0–1 cm), basal root (1–2 cm), and shoot for expression analysis. (B) Expression pattern of SlNAC063 in response to different metals. Seedlings with 3–4 cm root length were transferred to normal nutrient solution and the solution with 5 μM Al³⁺, 5 μM La³⁺, 1 μM Cu²⁺, and 5 μM Cd²⁺ for 6 h. RNA was extracted from the 0 to 1 cm root tips for expression analysis. (C) Time-course expression analysis. Tomato seedlings were treated with 5 μM Al³⁺ for 0, 0.5, 1, 3, and 6 h, respectively, and RNA was extracted from the 0 to 1 cm root tips for expression analysis. (D) Al dose-response expression analysis. Tomato seedlings were treated with nutrient solution containing 0, 5, 10, and 20 μM Al³⁺ for 6 h, respectively, and RNA was extracted from the 0 to 1 cm root tips for expression analysis. All treatment solution was 1/5 Hoagland nutrient solution (Pi, 10 μM; pH 5.0). Data are means ± SD (n = 3). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as an internal reference gene; the asterisk indicates significant differences between control and treatment at P < 0.05.