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. 2022 Mar 15;13:826954. doi: 10.3389/fpls.2022.826954

FIGURE 3.

FIGURE 3

Subcellular localization and transcriptional activation potential of SlNAC063. (A) Subcellular localization of SlNAC063. 35S::GFP and 35S::SlNAC063::GFP vectors were, respectively, expressed in tobacco leaves for 48 h, and then GFP signal was observed using a laser confocal microscope. Scale bars = 25 μm. (B) The C-terminus of SlNAC063 is responsible for its transcriptional activation activity. The left panel shows the pGBKT7 vectors containing different NAC063 protein fragments or the empty vector. The right panel shows the growth of yeast cells on SD with (+) or without (–) His medium with 4 mM 3-AT and X-α-gal at 30°C for 3 days. The appearance of the blue color was judged as positive results. (C) The C2 domain of SlNAC063 C-terminus is critical for its transcriptional activation activity. The left panel shows schematic diagram of the recombined vectors with different SlNAC063 protein fragments. The right panel shows the growth of yeast cells on SD with (+) or without (–) His medium with 4 mM 3-AT and X-α-gal at 30°C for 3 days.