FIGURE 7.

Expression levels of five selected genes in response to Al stress. (A–E) RT-qPCR analysis of gene expression of SlAAE3-1 (A), SlAAE3-2 (B), SlFDH (C), SlACS3 (D), and SlFRDL2 (E) in AC and SlNAC063 KO lines under Al stress. GAPDH was used as an internal control to normalize expression. Data are means ± SD (n = 3 for biological repeats). Different letters indicate significant difference (Tukey’s test, P < 0.5). (F) Correlation of gene expression levels between RNA-Seq data and real-time quantitative PCR (RT-qPCR) analysis used in (A–E) panels.