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. 2022 Mar 30;12:5375. doi: 10.1038/s41598-022-09306-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Human liver organoid cells expressed ACE2 and TMPRS2. (A) and (B) Feature plot and violin plot displaying single cell expression distributions for features (ACE2, TMPRSS2 and TMPRSS4) in each cluster of cells from liver organoids. (C) Co-immunofluorescence staining of human liver organoids for ACE2 and TMPRSS2. Parenthesis indicated the percentages of organoid cells that expressed ACE2 (i), TMPRSS2 (ii) and ACE2 plus TMPRSS2 (iii). Arrowheads, organoid cells that co-expressed ACE2 and TMPRSS2.