Figure 4.

Viral infection and replication kinetics of SARS-CoV-2 and SARS-CoV in human liver organoids. Bar chart showing viral titres in culture supernatants of human liver organoids infected with SARS-CoV-2 and SARS-CoV viruses. The organoids were infected at MOI 0.1 at 37 °C. Culture supernatants were harvested at the indicted times and virus titres were measured by TCID₅₀ assay. Bar-charts show the mean virus titre ± standard deviation (SD). The horizontal dotted line denotes the limit of detection in the TCID₅₀ assay. Statistical significances compared among viruses were calculated using two-way ANOVA with Tukey’s multiple comparisons test. ***p < 0·001. (C) Bar-charts showing the area under the curve (AUC) derived from the viral titres from 24 to 72 h. Data are presented as mean ± standard deviation (SD). Statistical significances compared among viruses were calculated using one-way ANOVA with Tukey’s multiple comparisons test. ***p < 0·001.