Figure 2.

Lung innate lymphoid cells (ILC) and dendritic cells (DC) in LPS-challenged and control mice. BALB/c mice were endonasally challenged with LPS (2 µg in 50 µl saline) or saline on four consecutive days ( Supplementary Figure 1A ). Twenty-four hours after the last challenge with LPS, lung tissue was resected for cell isolation and the different innate lymphoid cell (ILC) and dendritic cell (DC) subsets were identified by staining with mAbs and flow cytometric analysis. (A) ILC population (CD45⁺ Lin^- CD90.2⁺ CD127⁺ cells). (B) ILC1 (CD45⁺ Lin^- CD90.2⁺ CD127⁺ KLRG1^- RORγT^- NKp46⁺). (C) ILC2 (CD45⁺ Lin^- CD90.2⁺ CD127⁺ KLRG1⁺ RORγT^- NKp46^-). (D) NCR^- ILC3 (CD45⁺ Lin^- CD90.2⁺ CD127⁺ KLRG1^- RORγT⁺ NKp46^-). (E) NCR⁺ ILC3 (CD45⁺ Lin^- CD90.2⁺ CD127⁺ KLRG1^- RORγT⁺ NKp46⁺). (F) DC population (CD45⁺ CD11c⁺ MHCII⁺). (G) pDC (CD45⁺ CD11c⁺ MHCII⁺ Siglec H⁺ CD11b^-). (H) moDC (CD45⁺ CD11c⁺ MHCII⁺ CD64⁺). (I) CD11b^- CD103⁺ DC (CD45⁺ CD11c⁺ MHCII⁺ CD11b^- CD103⁺). (J) CD11b⁺ CD103^- DC (CD45⁺ CD11c⁺ MHCII⁺ CD11b⁺ CD103^-). ILC (total and subpopulations) are shown in absolute numbers of cells recovered from one left lung lobe in saline-treated mice (n = 8) and LPS-treated (n = 8). The total DC and subpopulations are expressed as % of live CD45+ cells in saline-treated mice (n = 5) and LPS-treated mice (n = 5). All data are represented as individual values and median with interquartile range. The Mann–Whitney test was used to calculate the p-values. *p < 0.05; **p < 0.01; ****p < 0.0001.