Table 2.
Lung cytokine production in lung tissue of wild-type BALB/c mice, SCID mice, and Rag2-/- γC-/- mice nasally exposed to saline or LPS.
| WT mice | SCID mice | Rag2-/- γC-/- mice | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Saline | LPS | p-value | Saline | LPS | p-value | Saline | LPS | p-value | |
| IL-1β | 0.33 (0.19 – 0.49) | 48.6 (23.8 – 71.3) | 0.0011 | 0.20 (0.16 – 0.28) | 27.7 (18.5 – 44.5) | <0.0001 | 25.6 (20.6 – 36.6) | 357 (156.7 – 629.6) | 0.147 |
| IL-17A | 0.8 (0.5 – 1.2) | 32.2 (15.9 – 93.9) | 0.0002 | 0.9 (0.6 – 1.2) | 6.2 (4.7 – 13.3) | 0.0041 | 0.7 (0.4 – 0.9) | 1.3 (0.7 – 1.7) | 0.582 |
| KC | 22.9 (13.2 – 28.8) | 54.9 (36.7 – 68.4) | 0.0031 | 25.1 (23.3 – 33.6) | 48.4 (36.3 – 72.5) | 0.0054 | 26.3 (21.2 – 29.9) | 28.5 (26 – 31.2) | 0.717 |
| IFN-γ | 0.5 (0.3 – 0.7) | 72.7 (50.9 – 129) | 0.0044 | 0.2 (0.09 – 0.5) | 55.7 (32.7 – 179.9) | <0.0001 | 0.4 (0.3 – 0.6) | 0.7 (0.4 – 0.9) | >0.999 |
| GM-CSF | 1.5 (1.1 – 1.6) | 10.2 (9.3 – 16.3) | 0.0091 | 1.3 (1.01 – 1.5) | 15.9 (11.5 – 27) | <0.0001 | 1.1 (0.97 – 1.4) | 2.2 (1.7 – 2.9) | 0.055 |
| IL-4 | 0 (0 – 0) | 1.5 (1.4 – 2.8) | 0.0005 | 0 (0 – 0.2) | 1.4 (0.97 – 1.9) | 0.0011 | 0.5 (0.2 – 0.6) | 0.9 (0.7 – 1.4) | 0.292 |
| IL-5 | 0 (0 – 0.7) | 1.7 (0.3 – 2.3) | 0.0534 | 0 (0 – 0.98) | 1.6 (0.8 – 2.6) | 0.0436 | 0 (0 – 0) | 0 (0 – 0.6) | >0.999 |
| IL-13 | 11 (0 – 13.7) | 0 (0 – 6.2) | 0.6444 | 0 (0 – 0) | 11.5 (0 – 19.9) | 0.0183 | 0 (0 – 10.6) | 0 (0 – 12.3) | >0.999 |
| IL-33 | 4176 (3613 – 5143) | 12554 (8880 – 19341) | 0.0039 | 3641 (3256 – 3897) | 12761 (10435 – 14451) | <0.0001 | 4928 (4201 – 5919) | 12723 (6958 – 17313) | 0.156 |
Values are expressed as pg per mg lung tissue and presented as median with 25 and 75 percentiles between brackets. Kruskal – Wallis with Dunn’s post hoc test was used to calculate the p-value. LPS, lipopolysaccharide.