Skip to main content
. 2022 Mar 10;13:853818. doi: 10.3389/fphar.2022.853818

FIGURE 2.

FIGURE 2

KCF18 downregulates cytokine-induced mRNA transcripts of TNF-α, IL-1β, and IL-6 in cells. PMA-pretreated THP-1 cells (A–C) or HMEC-1 cells (D–F) were cultivated with either cytokines at a concentration of 20 ng/ml or cytokines pretreated with KCF18 for 1 h. Four hours later, total RNA was isolated, and cytokine mRNA levels were determined using qPCR assays. GAPDH cDNA was used as an internal control. Values are the mean ± SD of mRNA levels relative to those for GAPDH from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 versus control, and #p < 0.05, ##p < 0.01, ###p < 0.001 versus cells stimulated with cytokines in the presence of KCF18.