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. 2022 Mar 10;13:854127. doi: 10.3389/fpls.2022.854127

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Copyright © 2022 Kopecký, Scholten, Majka, Burger-Meijer, Duchoslav and Bartoš.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Molecular cytogenetic analysis of Allium cepa (A. cepa) × Allium roylei (A. roylei) hybrids. Mitotic cells of F2 (A) and backcross (BC1) (B) plants and meiotic cells of F1 hybrid (C–F) after genomic in situ hybridization (GISH). During meiosis, homoeologous chromosomes initiate pairing in zygotene [(C); so far unpaired segments indicated by arrow] with complete pairing in pachytene (D). During anaphase I, chromosomes segregate to opposite poles (E) with only rare bridges (arrow) forming diads in TI (F) with rare micronuclei (arrow). Total guide DNA (gDNA) of A. roylei was labeled with digoxigenin (green/yellow color) and sheared DNA of A. cepa was used as blocking DNA (red pseudocolor).