FIGURE 5.
Effect of SYQP on MPO and F4/80 expression levels in lung tissues and hematological changes of LPS-induced mice. Animal experiment grouping and treatment were as described above, after the mice were sacrificed, the lung tissues of the mice were taken to make paraffin sections. (A,B) immunohistochemistry was used to label the expression levels of MPO. (C,D) immunofluorescence assay was used to label the macrophages. Red fluorescence represents F4/80-labeled macrophages and blue fluorescence represents DAPI-labeled nucleus. (E–I) The blood was collected in EDTA-K2 contained centrifugal tubes and subjected to automatic blood cell analyzer for detailed hematological analysis: (E) WBC, (F) neutrophils cells, (G) lymphocytes cells, (H) neutrophil-to-lymphocyte ratio and (I) platelets in LPS-induced ARDS mice. The bars represent the mean ± SD (n = 6); ####p < 0.0001, ###p < 0.001 and ##p < 0.01 versus the control group; ****p < 0.0001, ***p < 0.001, **p < 0.01 and *p < 0.05 versus the model group.