Fig. 2.

Evaluation of targeting and accumulating ability of GBM-sEVs after saponin treatment. (A) CD63, CD9 and ITGB1 detected by WB. (B) Representative histogram of DiO fluorescence in U87 and U251 cells. (C and D) Confocal fluorescence microscopic images of U87 and U251 cells. Scale bar: 10 μm. (E) In vivo fluorescence images of the orthotopic brain tumor model by an IVIS Spectrum/CT imaging system (upper). Statistical analysis of the DiR fluorescence in vivo (lower). (F) Fluorescence images of the main organs of the orthotopic brain tumor bearing mice were captured 24 h after DiR-labeled sEVs were intravenously injected (upper). Statistical analysis of the DiR fluorescence of the main organs (lower). *P < 0.05, ***P < 0.001, ns indicates no statistical significance.