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. 2022 Mar 16;16:204–217. doi: 10.1016/j.bioactmat.2022.02.013

Fig. 3.

Fig. 3

Quantitative proteomic analysis and qPCR analysis of U251-sEVs with and without saponin treatment. (A) Comparison of the most abundant 50 proteins between U251-sEVs and sa-U251-sEVs. (B) Comparison of the CD family and integrin family between U251-sEVs and sa-U251-sEVs. (C) The down regulated proteins in U251-sEVs after saponin detected by quantitative proteomic analysis. (D) The protein expression of ECM1, ANXA5, Alix, Tsg101 and Actin in U251-sEVs and sa-U251-sEVs detected by WB. (E) The mRNA expression of CD63, GFAP, GAPDH, VEGF, EGFR, TGFB1, IDH1, TIMP1, TIMP2, ERCC1, ERCC2 ABCC3, APNG, BIRC5, FGF2 were detected by qPCR, and the expression of let-7a, miR-15b, miR-16, miR-19b, miR-20, mir-21, miR-92, miR-320, miR-451a and miR-1246 were detected by qPCR. ***P < 0.001.