Figure 2.

Macrophages confer host protection in WT mice compared with the ATF3 KO. (A) Tissue sections of lung tissues stained with hematoxylin and eosin. (B) Pathological damage scores of lung tissues. (C) Measures of MPO activity as quantified by ELISA in lung extracts from WT or ATF3 KO mouse after S. aureus infection. (D) Representative images of WT and ATF3 KO mice lung tissues were assessed for neutrophils (S100A9) after S. aureus infection. (E) Representative zebra plot showing CD11b+ Ly6G+ cells from WT or ATF3 KO mice lung tissues after S. aureus infection. (F) Mice BALF was collected; counts of total cells are shown. WT and ATF3 KO mice were infected intratracheally with S. aureus (5 X 10⁷ CFU/mouse). After 6h, the mice were euthanized and their BALF was collected, stained and subjected to flow cytometry. (G) Representative images of WT and ATF3 KO mice lung tissues were assessed for macrophage (F4/80) after S. aureus infection. (H) Representative dot plot showing CD11b+ F4/80 + cells. (I) Quantification of (H) Data are shown as the mean ± SD. *P < 0.05; ***P < 0.001 by Student’s t test.