KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-HIV-1 p24 Monoclonal | NIH AIDS Reagent Program | Cat#3537 |
| Anti-HIV-1 SF2 p24 Polyclonal | NIH AIDS Reagent Program | Cat#4250 |
| Goat antiserum against NCp7 | Robert Gorelick, Institute National Cancer | N/A |
| Rabbit anti-DDX6 | Bethyl Laboratories | Cat#A300–461A, RRID:AB_2277216 |
| Rabbit anti-G3BP1 | Imed Gallouzi, McGill University | Gallouzi et al., 1998 |
| Goat anti-G3BP1 | Santa Cruz Biotechnology | Cat#sc-70283, RRID:AB_2107230 |
| Goat anti-TIAR | Santa Cruz Biotechnology | Cat#sc-1749,RRID:AB_632508 |
| Rabbit anti-Renilla Luciferase | MBL International | Cat#PM047, RRID:AB_1520866 |
| Sheep anti-Digoxigenin-AP, Fab fragments | Sigma | Cat#11093274910, RRID:AB_514497 |
| rabbit anti-phospho-eIF2α | Cell Signaling Technology | Cat#9721, RRID:AB_330951 |
| rabbit anti-eIF2α | Cell Signaling Technology | Cat#9722, RRID:AB_2230924 |
| rabbit anti-cleaved caspase-3 | Cell Signaling Technology | Cat#9661, RRID:AB_2341188 |
| rabbit anti-beta Actin | Abcam | Cat#ab8227, RRID:AB_2305186 |
| rabbit anti-GFP | Novus Biologicals | Cat#NB600–308 |
| Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor® 488 | Invitrogen | Cat#A-21202, RRID:AB_10003058 |
| Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor® 488 | Invitrogen | Cat#A-21206, RRID:AB_2535792 |
| Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor® 594 | Invitrogen | Cat#A-21207, RRID:AB_141637 |
| Donkey anti-Sheep IgG (H+L) Cross-Adsorbed, Alexa Fluor® 594 | Invitrogen | Cat#A-31573, RRID:AB_2716768 |
| Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed, Alexa Fluor® 647 | Invitrogen | Cat#A-31573, RRID:AB_2536183 |
| Donkey anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor® 647 | Invitrogen | Cat#A-21447, RRID:AB_141844 |
| goat anti-mouse IgG (H+L) Polyclonal Antibody (HRP (Horseradish Peroxidase)) | Rockland Immunochemicals | Cat#ROCK610–1319, RRID:AB_219659 |
| goat anti-rabbit IgG (H+L) Polyclonal Antibody (HRP (Horseradish Peroxidase))l | Rockland Immunochemicals | Cat#ROCK611–1322, RRID:AB_219723 |
| donkey anti-goat IgG (H+L) Polyclonal Antibody (HRP (Horseradish Peroxidase)) | Rockland Immunochemicals | Cat#ROCK711–703, RRID:AB_840935 |
|
Chemicals, Peptides, and Recombinant Proteins | ||
| HIV-1/NL4–3 (NCp7-WT) | Robert Gorelick, National Cancer Institute | Wu et al., 1996 |
| HIV-1/NL4–3 (NCp7-SSHS/SSHS mutant) | Robert Gorelick, National Cancer Institute | Guo et al., 2000 |
| HIV-1/NL4–3 (NCp7-linker mutant) | Robert Gorelick, National Cancer Institute | Wu et al., 2014a |
| EIAV (NCp11-WT) | Robert Gorelick, National Cancer Institute | Stewart-Maynard et al., 2008 |
| FIV (NCp10-WT) | Robert Gorelick, National Cancer Institute | Wu et al., 2014b |
| HTLV-1 (NCp15-WT) | Robert Gorelick, National Cancer Institute | Stewart-Maynard et al., 2008 |
| MuLV (NCp10-WT) | Robert Gorelick, National Cancer Institute | Stewart-Maynard et al., 2008 |
| RSV (NCp12-WT) | Robert Gorelick, National Cancer Institute | Stewart-Maynard et al., 2008 |
| SIV/Mne (NCp8-WT) | Robert Gorelick, National Cancer Institute | Post et al., 2016 |
| HIV-1 Gag protein | Abcam | Cat#ab109969 |
| FluoZin-3, AM, cell permeant | Thermo Fisher Scientific | Cat#F24195 |
| Alexa Fluor 488 Microscale Protein Labeling Kit | Thermo Fisher Scientific | Cat#A30006 |
| DMEM | GIBCO / Thermo Fisher Scientific | Cat#11965–118 |
| RPMI 1640 | Invitrogen | Cat#11875–119 |
| FBS | Wisent | Cat#080–150 |
| Penicillin-streptomycin | Wisent | Cat#450–201-EL |
| HEPES | VWR | Cat#CA-EM5320 |
| Ficoll (Lymphocyte Separation Medium) | Corning | Cat#25–072-CV |
| Dextran | Sigma | Cat#1179741 |
| JetPrime (PolyPlus) | VWR | Cat#CA89129–924 |
| Sodium Arsenite (NaAsO2) | Sigma | Cat#S7400 |
| TPEN (N,N,N′,N′-Tetrakis(2-pyridylmethyl) ethylenediamine) | Sigma | Cat#P4413 |
| 1,6-hexanediol | Sigma | Cat#240117 |
| Saquinavir | NIH AIDS Reagent Program | Cat#4658 |
| Trypsin/EDTA | Thermo Fisher Scientific | Cat#25200056 |
| Pluronic F-127 | Thermo Fisher Scientific | Cat#P3000MP |
| Poly-L-lysine solution 0.1% (w/v) in H2O | Sigma | Cat#P8920 |
| D-PBS, 1X | Wisent | Cat#311–425-CL |
| Formamide | VWR | Cat#CAFX0420–8 |
| Sodium Chloride | EMD Chemicals | Cat#Sx0420–5 |
| Tris | Bioshop Canada | Cat#TRS001.5 |
| EDTA-free Protease inhibitor tablets | Roche | Cat#11873580001 |
| Paraformaldehyde | Polysciences, Inc. | Cat#18814 |
| tRNA | Invitrogen | Cat#15401–021 |
| Triton-X | OmniPur, EMD Millipore | Cat#9400 |
| Denhardt’s Solution (50X) | Thermo Fisher Scientific | Cat#750018 |
| DNasel | Invitrogen | Cat#18047–019 |
| RNase OUT | Invitrogen | Cat#10777019 |
| Fluorescent Antibody Enhancer Set for DIG Detection #4 (blocking solution) | Roche | Cat#1768506 |
| ProLong Gold Antifade Mountant with DAPI | Invitrogen / Thermo Fisher Scientific | Cat#P36931 |
| DIG RNA Labeling Kit | Sigma | Cat#11277073910 |
| Transcription T7 RNA Polymerase | Invitrogen | Cat#18033–019 |
|
Critical Commercial Assays | ||
| Pierce ECL Plus western blotting Substrate | Thermo Fisher Scientific | Cat#32132 |
| Bradford protein assay | BioRad | Cat#500–0006 |
| HIV-1 p24 Antigen Capture Assay ELISA kit | Advanced Biosciences Laboratories | Cat#5421 |
|
Deposited Data | ||
| HIV-1 | NCBI protein sequence database | GenBank: AD39400.1 |
| SIV | NCBI protein sequence database | GenBank: AEK79593.1 |
| FIV | NCBI protein sequence database | GenBank: AA48157.1 |
| EIAV | NCBI protein sequence database | GenBank: ACT31322.1 |
| HTLV-1 | NCBI protein sequence database | GenBank: AAB20767.1 |
| RSV | NCBI protein sequence database | GenBank: AA48534.1 |
| MuLV | NCBI protein sequence database | RefSeq: NP_040332. |
| HTLV-2 | NCBI protein sequence database | GenBank: AA73488.1 |
| BLV | NCBI protein sequence database | GenBank: AAA42784.1 |
| MVV | NCBI protein sequence database | GenBank: AAB25459.1 |
| HIV-2 | NCBI protein sequence database | GenBank: AAA76840.1 |
| ALV | NCBI protein sequence database | GenBank: AJG42160.1 |
| SRV | NCBI protein sequence database | GenBank: AD89356.1 |
| MMTV | NCBI protein sequence database | GenBank: AAF31472.1 |
| FFV | NCBI protein sequence database | GenBank: GC11912.1 |
| SFVagm | NCBI protein sequence database | RefSeq: YP_001956721.2 |
| SFVcpz | NCBI protein sequence database | GenBank: AAA19977.1 |
| HFV | NCBI protein sequence database | GenBank: CAA68998.1 |
| FeLV | NCBI protein sequence database | GenBank: AAA43054.1 |
| GALV | NCBI protein sequence database | GenBank: ALV83305.1 |
| PERV | NCBI protein sequence database | GenBank: CAA76581.1 |
| BFV | NCBI protein sequence database | GenBank: AWK77106.1 |
| SnRV | NCBI protein sequence database | RefSeq: NP_043925.1 |
| WDSV | NCBI protein sequence database | RefSeq: NP_045938.1 |
| HERV-W | NCBI protein sequence database | GenBank: AAF74213.1 |
| HERV-K | NCBI protein sequence database | GenBank: CAA69289.1 |
| JSRV | NCBI protein sequence database | GenBank: CAA01899.1 |
| TIA1 | NCBI protein sequence database | GenBank: EAW99824.1 |
| hnRNPA2B1 | NCBI protein sequence database | RefSeq: XP_005249786.1 |
| hnRNPA0 | NCBI protein sequence database | RefSeq: NP_006796.1 |
| hnRNPA1L2 | NCBI protein sequence database | RefSeq: NP_001011725.1 |
| DAZAP1 | NCBI protein sequence database | RefSeq: NP_061832.2 |
| RNApolII | NCBI protein sequence database | GenBank: AAT12524.1 |
| HEWL | NCBI protein sequence database | PDB: 1LSZ_A |
| BPT1 | NCBI protein sequence database | RefSeq: NP_001001554.2 |
| CALM1 | NCBI protein sequence database | GenBank: AAD45181.1 |
| UB | NCBI protein sequence database | GenBank: AAA36789.1 |
| REV | NCBI protein sequence database | GenBank: AYF56141.1 |
| CRM1 | NCBI protein sequence database | GenBank: CAA69905.2 |
| DDX1 | NCBI protein sequence database | RefSeq: NP_004930.1 |
| DDX11 | NCBI protein sequence database | RefSeq: NP_085911.2 |
| DDX17 | NCBI protein sequence database | GenBank: CAG30318.1 |
| DDX18 | NCBI protein sequence database | GenBank: CAG33341.1 |
| DDX21 | NCBI protein sequence database | RefSeq: NP_004719.2 |
| DDX24 | NCBI protein sequence database | RefSeq: NP_065147.1 |
| DDX3 | NCBI protein sequence database | GenBank: AAC34298.1 |
| DDX4 | NCBI protein sequence database | RefSeq: NP_077726.1 |
| DDX5 | NCBI protein sequence database | RefSeq: NP_001307524.1 |
| DHX36 | NCBI protein sequence database | GenBank: AAH36035.1 |
| DHX9 | NCBI protein sequence database | RefSeq: NP_001348.2 |
| Eif5A | NCBI protein sequence database | GenBank: AAH80196.1 |
| PIMT | NCBI protein sequence database | GenBank: AAH07501.1 |
| RanGTP | NCBI protein sequence database | GenBank: CAG29343.1 |
| RHA | NCBI protein sequence database | GenBank: AML93444.1 |
| RIP | NCBI protein sequence database | GenBank: AAH46349.1 |
| Sam68 | NCBI protein sequence database | RefSeq: NP_006550.1 |
|
Experimental Models: Cell Lines | ||
| HeLa (ATCC® CCL-2) | American Type Culture Collection | N/A |
| HEK293T/17 (ATCC® CRL-11268) | American Type Culture Collection | N/A |
| Jurkat, CE6–1 (ATCC® TIB-152) | American Type Culture Collection | N/A |
| TZM-bl | NIH AIDS Reagent Program | Cat#8129 (Platt et al., 2009; Takeuchi et al., 2008; Wei et al., 2002; Derdeyn et al., 2000; Platt et al., 1998) |
|
Recombinant DNA | ||
| pcDNA3.1 | Thermo Fisher Scientific | Cat#V79020 |
| HIV-1 NL4–3 Infectious Molecular Clone (pNL4–3) | NIH AIDS Reagent Program | Cat#114 |
| Gag-mCherry | Paul Bieniasz, Rockefeller University, USA | Jouvenet et al., 2008 |
| pG3BP1-GFP | Imed Gallouzi, McGill University, Canada | Tourriére et al., 2001 |
| pNL4–3 NC-C15S-C49S | Robert Gorelick, National Cancer Institute | Guo et al., 2000 |
| pRluc-N1 | BioSignal Packar | Cat#631000 |
| p2-p1/Rluc | Mouland Lab | Chatel-Chaix et al., 2004 |
| NC-p1R7-YFP | Mouland Lab | Chatel-Chaix et al., 2008 |
| NC-p1C15–49S-YFP | Mouland Lab | Chatel-Chaix et al., 2008 |
| pPR-EGFP | Rongtuan Lin, McGill University | Solis et al., 2011 |
| pEGFP-C1 | Clontech | Cat#6084–1 |
|
Software and Algorithms | ||
| Imaris software v. 8.1.2 | Oxford Instruments / Imaris | https://imaris.oxinst.com/ |
| Volocity v. 6.3 | Perkin Elmer | https://www.perkinelmer.com/ |
| ImageJ | National Institutes of Health (NIH) | https://imagej.nih.gov/ij/ |
| GraphPad v. 6.1 | Prism | https://www.graphpad.com/scientific-software/prism/ |
| Predictor of prion-like domains (PLAAC) | Lancaster et al., 2014 | http://plaac.wi.mit.edu/ |
| Predictor of Natural Disordered Regions (PONDR) | Molecular Kinetics, Inc.(Peng et al., 2006, 2005; Romero et al., 2001) | http://www.pondr.com/ |
| MobiDB: a database of protein disorder and mobility annotations | Piovesan et al., 2018 | http://mobidb.bio.unipd.it/ |
| Protein sequence analysis tool | Philipp O. Tsvetkov | Garnier, et al. (2017) |
|
Other | ||
| Leica DM16000B laser confocal microscope | Leica | N/A |
| WaveFX spinning disk confocal head | Quorum Technologies | N/A |
| Hamamatsu EM-CCD digital camera | Hamamatsu | N/A |
| Hybridization Oven | Boekel Scientific | Model 24100 |
| Nitrocellulose membranes 0.2 µm | Bio-Rad | Cat#1620112 |
| Quick Spin Columns | Roche | Cat#11814427001 |
| 4-chamber Lab-Tek®II Chambered #1.5 German Coverglass System | Thermo Fisher Scientific | Cat#155382 |
| 12-well plates | VWR | Cat#CA62406–165 |
| Boil proof 1.5 mL tubes | DIAMED | Cat#DIATE610–3167 |
| Eppendorf® micropestles | Sigma | Cat#Z317314 |
| 25 × 75 mm x 1mm thick glass slides | Thermo Fisher Scientific | Cat#640–004T |
| 18 mm ø No. 1 cover glasses | VWR | Cat#16004–300 |
| 0.2 µm Sterile syringe filters | VWR | Cat#28143–310 |