Fig. 1.

Fabrication and characterization of anti-Sca-1 antibody-functionalized vascular grafts. (A) Experimental schematic for immobilization of anti-Sca-1 antibodies on electrospun PCL vascular graft. (B) Images show the tubular vascular grafts (1 cm in length) and representative SEM images of the electrospun PCL and PCL-Sca-1 Ab grafts. Scale bar for SEM images, 30 μm. (C) Representative confocal laser scanning microscopy (CLSM) images revealed that anti-Sca-1 antibodies were modified on electrospun PCL grafts. Scale bar, 20 μm. (D) Representative stress-strain curves of the PCL and PCL-Sca-1 Ab grafts. (E) Surface hydrophilic/hydrophobic performance characterized by static water contact angle measurement. (F) The burst pressure of the PCL and PCL-Sca-1 Ab grafts. Data were expressed as mean ± s.e.m. for each group, n = 3 independent experiments. (G) Quantitative analysis of the human plasma fibrinogen (FGN) adsorption on the different mats measured by ELISA. Data were expressed as mean ± s.e.m. for each group: **p < 0.01. n = 6 independent experiments. (H) SEM images showed the platelet adhesion on the different mats. Scale bar, 10 μm. (I) Quantitative analysis of the adhered platelets. Data were expressed as mean ± s.e.m. for each group: **p < 0.01. n = 6 independent experiments.