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. 2022 Mar 16;9:844671. doi: 10.3389/fcvm.2022.844671

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Copyright © 2022 Wang, Xu, Wu, Shen, Lin, Fang, Zhang, Shen, Liu and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of parathyroid hormone (PTH) on COL1A1 expression in human umbilical vein endothelial cells (HUVECs). (A) After HUVECs were treated with vehicle control, 100 pM PTH, PTH + BTP2 (an Orai nonspecific inhibitor), PTH + CsA (calcineurin inhibitor), or PTH + W7 (calmodulin antagonist) for 24 h, COL1A1 mRNA levels were analyzed by qPCR assays. Messenger RNA expression was normalized to that of GAPDH. (B,C) Representative images (B) and summary data (C) showing COL1A1 protein expression in HUVECs after treatment with vehicle control, 100 pM PTH, PTH + BTP2, PTH + CsA or PTH + W7 for 24 h. GAPDH expression was referenced as the loading control. Data are shown as the mean ± SEM; n = 5–6. *P < 0.05, ****P < 0.0001 vs. Con or PTH in (A) and (C) analyzed by one-way analysis of variance followed by Dunnett's multiple comparisons test.