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In vitro cytocompatibility of various ZQ71 samples. (A) CLSM images of the cells stained by FDA and PI. (B) CLSM images of the cell cytoskeleton (red) and nucleus (blue) stained by rhodamine-phalloidin and DAPI. (C) Cell viability of MC3T3-E1 cultured with different ZQ71 samples at day 1, 2 and 3. (D–H) qRT-PCR analysis for several osteogenic gene expression of MC3T3-E1 cultured with different ZQ71 samples at day 3, 7 and 11. (*p < 0.05, **p < 0.01, ***p < 0.001)
