Fig. 1.

Experimental analysis of biochemical correlates and CaMKK2 expression in the piglet model of neonatal hypoxic brain injury. a ATP measurement in cerebral cortex tissue from piglets in the Nx (blue), Hx (red), and Hx+PP2 (green) groups; statistics: one-way ANOVA overall: F (2, 10) = 89.02 (P < 0.0001), Tukey multiple comparison test: Nx vs. Hx: P < 0.0001, Hx vs. Hx+PP2: P = 0.9253, Nx vs. Hx+PP2: P < 0.0001. b Nuclear Ca²⁺ influx in cerebral cortical tissue for all groups; statistics: one-way ANOVA overall: F (2, 14) = 32.94 (P < 0.0001), Tukey multiple comparison test: Nx vs. Hx: P < 0.0001, Hx vs. Hx+PP2: P = 0.0116, Nx vs. Hx+PP2: P = 0.0007. c Western blots of CaMKK2 for Nx, Hx, and Hx+PP2 groups, control: β-actin. d Quantification of CaMKK2 protein expression from western blots; statistics: one-way ANOVA overall: F (2, 15) = 19.68 (P < 0.0001), Tukey multiple comparison test: Nx vs. Hx: P = 0.0004, Hx vs. Hx+PP2: P = 0.0002, Nx vs. Hx+PP2: P = 0.9336