Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 29;19:25. doi: 10.1186/s12989-022-00462-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 3 — PM aggravated mitochondrial injury in endothelial cells treated with high glucose, and 1,25(OH)₂D₃ pretreatment reduced this change. HUVECs were pretreated with high glucose (30 mM) for 24 h and then treated with PM (50 μg/mL) for 8 h. HUVECs were pretreated with 100 nM 1,25(OH)₂D₃ for 12 h before PM exposure. A The JC-1 assay and fluorescence microscopy were used to examine the mitochondrial membrane potential. Bar = 50 μm. B, C The JC-1 assay and flow cytometry were used to examine mitochondrial membrane potential. Statistical analysis of the ratios of JC-1 monomer to JC-1 aggregates. D, E MitoStatus TMRE dye was used for the quantitative analysis of the mitochondrial membrane potential with flow cytometry. F The ATP assay was conducted to assess ATP levels. *P < 0.05 compared with the control group; ^†P < 0.05 compared with the HG group; ^#P < 0.05 compared with the PM group; ^§P < 0.05 compared with the HG + PM group