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. 2022 Mar 11;12:763464. doi: 10.3389/fonc.2022.763464

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Copyright © 2022 Mahajan, Gujral, Jain and Ponnampalam

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative TET1 TET2 and TET3 mRNA expression in response to steroid hormone treatment in AN3 cells. The y-axis shows the fold change of mRNA levels following different treatments compared with control, all results corrected against geo-mean expressions of three reference genes - YWHAZ, RPLO and RPL13a. The x-axis shows different treatment groups. E24 = 24h Estrogen; EP24, EP48 and EP72 = both Estrogen + Progesterone for 24, 48 and 72h. Data are presented as mean ± SEM *p < 0.05. P ≤ 0.1 was considered as approaching significance. The experimental setup included three independent sets of cell culture experiments (n = 3) and triplicates of each sample for the RT-PCR.