Figure 5.

Upregulation of hsa-miR-130b-3p decreased expression of PTEN via the predicted binding site. (a) PTEN was downregulated in the 15 CH patients compared with the 21 CS by qPCR analysis. (b) The predicted binding site of PTEN and hsa-miR-130b-3p. The white area indicates the binding site of PTEN and hsa-miR-130b-3p. (c, d) The normalized luciferase activity of PTEN following transfection with hsa-miR-130b-3p mimics and NC mimics. Values are represented as the mean ± SEM. n = 3; ^∗P < 0.05 and ^∗∗∗P < 0.001.