TABLE 3.
Putative candidate genes underlying the loci associated with GZnC and GFeC.
| Traita | Chrb | Physical interval of identified QTL (Mb)c | Candidate gened | Physical positionc | Distanced (Mb) | Ortholog/Putative functionalitye | Putative involved processf |
| GZnC | 1A | 515.79–516.03 | TraesCS1A01G326700 | 516.73 | 0.70 | Citrate-binding protein | Transport |
| 1A | 580.42–586.13 | TraesCS1A01G425100 | 580.18 | 5.95 | JAR1 (Kobayashi, 2019) | Regulations | |
| 3A | 19.89–22.61 | TraesCS3A01G036400 | 19.98 | 0.09 | ABC transporter G family member | Uptake | |
| 3A | 721.64–724.58 | TraesCS3A01G499300 | 724.49 | 0.09 | OsPEZ2 (Bashir et al., 2011; Ishimaru et al., 2011) | Uptake | |
| 3B | 202.41 | TraesCS3B01G191300 | 204.28 | 1.88 | OsYSL1 (Chu et al., 2010) | Transport | |
| 3B | 242.66 | TraesCS3B01G214000 | 253.90 | 11.24 | TaCNR5 (Qiao et al., 2019) | Transport | |
| 5A | 591.28–592.63 | TraesCS5A01G391700 | 587.40 | 5.24 | Vrn-A1 (Jobson et al., 2018) | Regulations | |
| 5D | 319.22 | TraesCS5D01G209900 | 318.10 | 1.12 | TaVIT2-5D (Connorton et al., 2017) | Storage | |
| 6A | 17.77 | TraesCS6A01G042900 | 22.54 | 4.76 | OsYSL7 (Chu et al., 2010) | Transport | |
| 6A | 28.31–31.37 | TraesCS6A01G051700 | 26.93 | 1.95 | NAC domain-containing protein | Regulations | |
| 6B | 142.73 | TraesCS6B01G145600 | 145.88 | 3.14 | ABC transporter B family protein | Uptake | |
| 6D | 27.52 | TraesCS6D01G049900 | 24.19 | 3.33 | OsYSL8 (Grillet and Schmidt, 2019) | Transport | |
| 7A | 706.91 | TraesCS7A01G527900 | 708.67 | 1.76 | Magnesium transporter | Uptake | |
| 7B | 533.78–540.78 | TraesCS7B01G299200 | 536.01 | 3.21 | BZIP transcription factor | Regulations | |
| 7B | 626.06–626.07 | TraesCS7B01G364800 | 627.98 | 1.91 | NRAMP1 (Segond et al., 2009) | Storage | |
| 7B | 687.32–689.92 | TraesCS7B01G429600 | 698.14 | 9.30 | Zinc ion binding protein | Storage | |
| 7B | 708.11 | TraesCS7B01G444600 | 708.95 | 0.83 | Magnesium transporter | Transport | |
| 7D | 203.16 | TraesCS7D01G237500 | 201.88 | 1.29 | Copper-transporting ATPase | Uptake | |
| 7D | 506.11 | TraesCS7D01G392800 | 507.81 | 1.70 | BZIP transcription factor | Regulations | |
| 7D | 605.17 | TraesCS7D01G504400 | 610.53 | 5.36 | Heavy metal transport | Transport | |
| GFeC | 1B | 15.65–15.74 | TraesCS1B01G030800 | 15.16 | 0.28 | ABC transporter ATP-binding protein | Uptake |
| 1B | 660.01 | TraesCS1B01G437100 | 659.98 | 0.03 | Calcium-transporting ATPase | Uptake | |
| 5B | 531.58 | TraesCS5B01G349500 | 530.78 | 0.80 | Fe2+ transporter | Transport | |
| 5B | 548.33 | TraesCS5B01G370100 | 548.81 | 0.47 | ATP-dependent zinc metalloprotease | Transport | |
| 5B | 622.54 | TraesCS5B01G453100 | 626.01 | 3.46 | OsYSL3 (Waters et al., 2006) | Transport | |
| 7D | 54.99 | TraesCS7D01G098100 | 58.90 | 3.90 | OsIRT2 (Ishimaru et al., 2006) | Uptake | |
| 7D | 69.31 | TraesCS7D01G113100 | 69.26 | 0.06 | BHLH family transcription factor | Regulations | |
| 7D | 614.51–614.92 | TraesCS7D01G515800 | 615.91 | 1.37 | Magnesium transporter | Transport |
aGZnC, grain zinc concentration; GFeC, grain iron concentration.
bChr, chromosome.
cPhysical positions of SNP markers and annotated genes were based on IWGSC RefSeq v.1.0.
dThe distances between representative markers and candidate genes.
eGene ID and functional annotations were based on IWGSC RefSeq v.1.0. The underlined genes indicated the cloned Zn/Fe-related genes in common wheat.
fPutative involved processes were based on the regulatory mechanisms of corresponding orthologous gene in Arabidopsis and rice, as well as gene annotations from IWGSC RefSeq v.1.0.