Fig 1. No evidence of heritability of neutralization breadth.

Distribution and heritability of peak neutralization breadth across individuals. Non-broad neutralizers (indicated in blue) are defined as individuals who neutralized <35% of a 34-virus panel three to four years post-diagnosis and broad neutralizers (indicated in red) as individuals who neutralized >70% of the panel. Individuals sampled less than two years post-infection (intermediate neutralizers) are also shown (indicated in lavender). (A) Boxplot of peak neutralization breadth across HIV-1 subtypes and recombinants in the RV217 cohort. The number of individuals in each group is shown in parenthesis. (B) Histogram of neutralization breadth measured at peak neutralization breadth (RV217, n = 70) and three years after infection (RV217, n = 32; RV144, n = 39); dots for each participant are shown alongside each histogram. (C) Ancestral reconstruction of neutralization breadth across a phylogeny reconstructed from consensus sequences from the earliest post-diagnosis samples in 70 RV217 individuals (filled circles) and 56 RV144 placebo participants (open circles). The color spectrum shows the reconstructed ancestral estimates for neutralization breadth. (D) Density plot of the H² (heritability) estimates from three conditioned runs (chains) of a phylogenetic Ornstein-Uhlenbeck mixed model performed on the phylogeny in (C). The solid line shows the mean of the posterior estimates of H².