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. 2022 Mar 14;10:817028. doi: 10.3389/fcell.2022.817028

FIGURE 2.

FIGURE 2

The silence of circSSU72 inhibited PTC cell proliferation, migration, and invasion. (A) The expression of circSSU72 was investigated in five PTC cell lines (n = 3). (B) TPC-1 and IHH-4 cells were transfected with siRNAs of circSSU72, and the expression level of circSSU72 was analyzed by qRT-PCR assay (n = 3). (C) Colony formation assay of the cell proliferation ability in TPC-1 and IHH-4 cells transfected with si-circSSU72 (n = 3). (D) CCK8 assay to assess the influence of circSSU72 on TPC-1 cell (n = 3). (E) CCK8 assay to assess the influence of circSSU72 on IHH-4 cell (n = 3). (F) EdU assay of the cell proliferation ability in TPC-1 cell transfected with si-circSSU72 (scale bar = 100 um, n = 4). (G) EdU assay of the cell proliferation ability in IHH-4 cell transfected with si-circSSU72 (scale bar = 100 um, n = 4). (H) Scratch wound healing assays in transfected TPC-1 cell (scale bar = 200 um, n = 3). (I) Scratch wound healing assays in transfected IHH-4 cell (scale bar = 200 um, n = 3). (J) Transwell invasion and migration assay in transfected TPC-1 cell (scale bar = 100 um, n = 3). (K) Transwell invasion and migration assay in transfected IHH-4 cell (scale bar = 100 um, n = 3). p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.