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. 2022 Mar 14;10:817028. doi: 10.3389/fcell.2022.817028

FIGURE 6.

FIGURE 6

miR-451a inhibited the proliferation, migration, and invasion of PTC cells by targeting S1PR2. (A) Green fluorescent showing the successful transfection of S1PR2 overexpression vectors in TPC-1 and IHH-4 cells (scale bar = 200 um). (B) The efficacy of miR-451a mimics was reconfirmed in TPC-1 cell with the transfection of S1PR2 vectors and NC vectors (n = 3). (C) The expression of S1PR2 in TPC-1 cell with the transfection of S1PR2 vectors miR-451a mimics (n = 3). (D) The efficacy of miR-451a mimics was reconfirmed in IHH-4 cell with the transfection of S1PR2 vectors and NC vectors (n = 3). (E) The expression of S1PR2 in IHH-4 cell with the transfection of S1PR2 vectors miR-451a mimics (n = 3). (F) The expression of S1PR2 protein in transfected TPC-1 cell by western blot assay. (G) CCK8 assay to assess the influence of miR-451a and S1PR2 on TPC-1 cell (n = 3). (H) CCK8 assay to assess the influence of miR-451a and S1PR2 on IHH-4 cell (n = 3). (I) EdU assay of the cell proliferation ability in TPC-1 cell transfected with miR-451a mimics and S1PR2 vectors (scale bar = 100 um, n = 3). (J) Transwell invasion and migration assay in transfected TPC-1 cell (scale bar = 100 um, n = 3). (K) The expression of AKT, p-AKT proteins in transfected TPC-1 cell by western blot assay.*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.