FIGURE 3.
Capsaicin alleviates UVB-induced decrease on collagen in cultured fibroblasts. The dermal fibroblasts were isolated from postnatal day 2 mice and cultured in vitro. Cells were incubated with capsaicin (Cap) from 1 to 10 μM with or without UVB irradiation at 300 mW/m2 for 5 min. (A) The mRNAs of COL1A1, COL1A2, and COL3A1 were determined using qRT-PCR with (left panel) or without (right panel) UVB. (B) The protein lysates were collected for SDS-PAGE and Western blot using antibody against COL1α1 (∼138 kDa) and α2 (∼129 kDa) with (left panel) or without (right panel) UVB. Antibody against α-tubulin (∼55 kDa) was used as a loading control. TGF-β1 (5 ng/ml) was used as a positive control here. The quantification of the blots was shown below (bottom panels). Values are in percentage of change, or increase, against control, in mean ± SD, n = 5, each with triplicate samples. *, p < 0.05; **, p < 0.01; ***, p < 0.001 compared with control (vehicle only).