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. 2022 Mar 30;8(13):eabj8658. doi: 10.1126/sciadv.abj8658

Fig. 1. Neuronal C/EBPβ overexpression elicits short life span, associated with behavioral impairments in Thy1-C/EBPβ Tg mice, related to fig. S1.

Fig. 1.

(A) Human C/EBPβ overexpression in neurons shortens life span. C/EBPβ Tg strain of origin: C57BL/6; P < 0.0001, log-rank test; ***P < 0.001. (B) C/EBPβ knockdown extends life span. C/EBPβ+/− strain of origin: 129S1/Sv-Oca2+ Tyr+ Kitl+; P < 0.0001, log-rank test; ***P < 0.001. (C) Left: Images from PET-CT scanning of 18F-FDG uptake in 6-month-old Thy1-C/EBPβ Tg, C/EBPβ+/−, and age-matched WT littermates. Right: Standardized uptake value (SUV) at increasing time intervals after injection of 18F-FDG. Means ± SEM; n = 3 mice per group; **P < 0.01; Mann-Whitney U test. (D) Seizure duration after administration of PTZ (35 mg kg−1). Control (WT), n = 6; C/EBPβ Tg, n = 6; C/EBPβ+/−, n = 6 mice. *P < 0.05 and **P < 0.01; Mann-Whitney U test. (E) Elevated [Ca2+]i in the C/EBPβ-overexpressing hippocampal CA1. The box plots indicate first, median, and third quartiles as well as outliers. N = 196 (WT control) and 95 cells (C/EBPβ Tg) pooled from six animals each; *P = 0.043, t(289) = 2.04; two-tailed Student’s t test. (F) PPI. Means ± SEM; n = 8 mice per group; unpaired t test with Welch’s correction. (G) Open field test. Means ± SEM; n = 8 mice per group; *P < 0.05; unpaired t test with Welch’s correction. (H) FST. Means ± SEM; n = 8 mice per group; unpaired t test with Welch’s correction. (I) Sleep latency test. Means ± SEM; n = 8 mice per group; *P < 0.05; unpaired t test with Welch’s correction. (J and K) MWM analysis of cognitive functions. Means ± SEM; n = 8 mice per group; *P < 0.05 and **P < 0.01; unpaired t test with Welch’s correction. (L) Fear condition tests. Means ± SEM; n = 8 mice per group; *P < 0.05, and **P < 0.01; unpaired t test with Welch’s correction.