Figure 2.

Biofilm thickness of F. nucleatum subspecies determined by CLSM. (A) Representative Z-stack 3D images of single-subspecies biofilms grown on poly-L-lysine coated plastic surface. Biofilms are enclosed in a bounding box with scaled coordinates; x, y, and z axes show the dimensions indicated in μm. Differences in biofilm thickness can be observed. (B) Biofilm thickness estimated from z-stacks. Experiment was performed once with biofilms grown in triplicates. Mean values with standard deviations are shown.