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Folding and locations of the various constructs. Immunodetections were carried out with two monoclonal antibodies directed against epitopes located on the monomeric form (rows A and B), with a monoclonal antibody specific to a trimeric epitope (C), and with polyclonal antibodies directed against cell envelope (D). Lanes: 0, E. coli BZB1107, devoid of porin; WT, BZB1107 (pLG361), encoding the wild-type OmpF; 119D, 119E, 132A, and 132D, BZB1107 expressing the various mutants.
