Table 1.
Details of histologic assessment
| Histological Features | Staining Intensity | |||
|---|---|---|---|---|
| 0 | 1+ | 2+ | 3+ | |
| Light microscopy a | ||||
| Neutrophil infiltration | Absent | Mild (<25% of glomeruli) |
Moderate (25%–50% of glomeruli) |
Severe (>50% of glomeruli) |
| Endocapillary hypercellularity | ||||
| Hyaline deposits | ||||
| Cellular/fibrocellular crescents | ||||
| Fibrous crescents | ||||
| Fibrinoid necrosis | ||||
| Glomerulosclerosis | ||||
| Interstitial inflammation | Absent | Mild (<25% of the cortex) |
Moderate (25%–50% of the cortex) |
Severe (>50% of the cortex) |
| Interstitial fibrosis | ||||
| Tubular atrophy | ||||
| Immunofluorescence staining b | ||||
| IgG, IgA, IgM, C1q, C3, fibrinogen | Absent | Present | Present | Present |
Staining intensity was scored on a 0 to 3+ scale; deposits were considered present when the intensity was >1.
a
Specimens were fixed in 5% formalin. Stains used were hematoxylin and eosin, Periodic acid–Schiff, silver methenamine, and Masson trichrome/Acid Fuchsin Orange G.
b
Formalin-fixed, paraffin-embedded kidney sections incubated with pronase (13).