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. 2021 Nov 3;3(1):51–62. doi: 10.34067/KID.0003802021

Figure 1.

Figure 1.

Shroom3Gt/+ mice exhibit worse ischemia reperfusion kidney injury (IRI)–induced tubular injury (A) Immunofluorescence (IF) demonstrating Shroom3 expression primarily in cortical tubular epithelium (arrowhead). (B) Shroom3 expression continues to be expressed in cortical tubules 48 hours and 10 days after IRI (arrowhead). The levels of Shroom3 expression are reduced in Shroom3Gt/+ tubules but the spatial pattern remains identical. (C) and (D) Shroom3Gt/+ mice have higher mortality rates after a 28-minute and 22-minute IRI. (E)–(L) Representative H&E-stained kidney tissue 48 hours and 10 days after IRI. After 48 hours Shroom3Gt/+ kidneys exhibit more severe cortical and medullary tubular damage (arrowheads) when compared with WT (E)–(H). After 10 days the Shroom3Gt/+ kidneys exhibit severe histopathology although the WT mice have recovered (I)–(L). (M) Analysis of kidney function. Wild-type mice show a two-fold increase in serum creatinine levels at 24 hours after IRI, P=0.02 (*), which returned to baseline levels by 48 hours. In contrast, Shroom3Gt/+ mice show a 3.8-fold increase in serum creatinine levels after 24 hours, P<0.0001 (**), which persisted at 48 hours after IRI, P<0.0001 (***) and trended to baseline levels by day 10. Scale bars = 400 µm (A), 10 µm (B), 200 µm (E)–(L). WT = wild-type.