Table 2.
Conditions used to model ischemia
| Condition (Abbreviation) | Incubator-Oxygen Tension | Perfusion | Glucose and Nutrient Availability |
|---|---|---|---|
| N+P+glu | Normoxia (21% O2) | Yes (rocker) | Coculture medium |
| N+P-glu | Normoxia (21% O2) | Yes (rocker) | DMEM w/o glucose |
| N+S+glu | Normoxia (21% O2) | No (static) | Coculture medium |
| N+S-glu | Normoxia (21% O2) | No (static) | DMEM w/o glucose |
| L+P+glu | Low oxygen (5% O2) | Yes (rocker) | Coculture medium |
| L+P-glu | Low oxygen (5% O2) | Yes (rocker) | DMEM w/o glucose |
| L+S+glu | Low oxygen (5% O2) | No (static) | Coculture medium |
| L+S-glu | Low oxygen (5% O2) | No (static) | DMEM w/o glucose |
N, normoxia; L, low oxygen; P, perfusion; S, static; +glu, coculture medium; -glu, DMEM w/o glucose.