Fig. 4.

MnTE-2-PyP maintained mitochondrial homeostasis after radiation and hyperglycemia stress in normal prostate fibroblasts. Human prostate fibroblast cells were treated with 20 mM glucose (HG) and 30 μM MnTE-2-PyP (T2E) followed by 3 Gy of X-rays (RAD). 5 days after radiation, cells were harvested. Mitochondrial and cytosolic proteins were extracted. A. Representative western blots for DRP1 (mitochondrial fission protein), MFN1, MFN2 and OPA1 (mitochondrial fusion proteins) were measured and quantified. B. Quantification of PGAM5 in the cytosolic protein extract were measured. C. Cells were harvested at 30 h and 5 days post radiation. PINK1 protein levels were quantified in mitochondrial protein extracts. All images and graphs are representative of at least 3 independent experiments. CON = control, HG = high glucose, T2E = 30 μM MnTE-2-PyP treated, TG = T2E + HG treated, RAD = 3 Gy X-ray treated, RG = RAD + HG treated, RT = RAD + T2E treated and RGT = RAD + HG + T2E treated.

(*), ($), (#) and (@) denote a significant difference (p ≤ 0.5) as compared to control, HG, RAD and RAD + HG group respectively.