Fig. 6.

MnTE-2-PyP-mediated mitochondrial protection is prior to inhibition of myofibroblast differentiation after radiation and hyperglycemia stress in normal prostate fibroblasts. Human prostate fibroblasts were treated with 20 mM glucose (HG) and 30 μM MnTE-2-PyP (T2E) followed by 3 Gy of X-rays (RAD). 1–5 days post-radiation, cells were subjected to immunofluorescence assay with mitochondrial ATP synthase (red) and α-SMA (green) antibody. A. Representative image of human prostate fibroblasts stained with mitochondrial ATP synthase (red), α-SMA (green) antibody and DAPI (blue), 5 days after radiation. Scale bar = 100 μm. B. Intensity of mitochondrial ATP synthase per cell over time was quantified by Image J. C. Differences between the slopes of the lines were measured by linear regression analysis of the means of the intensity values using GraphPad Prism. D. Percentage of α-SMA stress fiber containing cells over time was quantified by Image J. E. Differences between the slopes of the lines were measured by linear regression analysis of the means of the intensity values using GraphPad Prism. All data is representative of at least 3 independent experiments. (*) denotes a significant difference (p ≤ 0.5) as compared to RAD + HG group. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)