MnTE-2-PyP-mediated restoration of altered metabolic and lipid profile in radiated human prostate fibroblasts. Human prostate fibroblast cells were treated with 30 μM MnTE-2-PyP (T2E) followed by 3 Gy of X-rays (RAD). 5 days after radiation, cells were harvested, and subjected to metabolomics and lipidomics analyses. A. Enriched pathways, analyzed from altered metabolites through RAD treatment, were analyzed by MetaboAnalyst and are displayed with fold enrichment. B. Representative metabolite changes from the CON, RAD, and RAD + T2E (RT) treatment data sets. (*) Denotes p-values < 0.05 and VIP >1. C. Enriched pathways, analyzed from altered lipids through RAD treatment, were analyzed by MetaboAnalyst and are displayed with fold enrichment. D. Representative lipid changes from the CON, RAD, and RAD + T2E (RT) treatment data sets. (*) Denotes p-values < 0.05 and VIP >1. CON = control, RAD = 3 Gy X-ray treated and RT = RAD + T2E treated cells. (*) and (#) denote a significant difference (p ≤ 0.5) as compared to control and RAD group respectively. All data is representative of at least 5 independent experiments.