Extended Data Fig. 5. Absolute values of pro-inflammatory and suppressive cytokines in pg/mL and anakinra levels measured by IL1-Ra in CSF and serum.
a, Time-course of anti-inflammatory agents (tocilizumab, anakinra and corticosteroids) administered to patients following CAR T cell infusions represented graphically to provide context for interpreting cytokine levels at various timepoints. b–d, The same data in absolute values that is represented in heatmap form in Fig. 4. Each data point represents the pg/ml value of the indicated cytokine at the timepoint following GD2-CAR T cell infusion. b, Pro-inflammatory cytokine levels in blood and cerebrospinal fluid (CSF) following intravenous (IV) administration. N = 4 patients (blood) and 3 patients (CSF). c, Pro-inflammatory cytokine levels in blood and cerebrospinal fluid (CSF) following intra-cerebroventricular (ICV) administration. N = 3 patients. d, Immune-suppressive TGFβ cytokine levels in the CSF following IV CAR T cell administration. N = 3 patients. e, Peak serum and CSF IL1-Ra levels during anakinra treatment. Anakinra is recombinant IL1-Ra. CSF levels of anakinra, which can cross the blood-brain barrier, were approximately one tenth those of serum levels. Data generated by Luminex multi-plex cytokine analysis of patient blood plasma and CSF samples. N = 3 patients. b–e, Each data point represents the mean of two technical replicates. Red=DIPG-1, Blue=DIPG-2, Purple= DIPG-3, Green=Spinal DMG-1. Filled dot=IV, open dot=ICV.