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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: Lab Invest. 2021 Dec 21;102(4):363–375. doi: 10.1038/s41374-021-00697-0

Figure 1. SFB does not colonize or induce immune responses in oral mucogingival tissues.

Figure 1.

A. Experimental timeline. Eleven-week-old male GF and SFB mice were euthanized, and specimens harvested for analysis. B-C. 16S rDNA analysis for SFB colonization; relative quantification of rDNA was performed via the CT method (2−ΔCT). B. 16S rDNA analysis of SFB expression in distal ileum (n=5/gp). C. 16S rDNA analysis for SFB colonization in gingiva (n=5/gp). D-E. Il17a mRNA qRT-PCR analysis; relative quantification of mRNA was performed via the 2−ΔΔCT method. D. Il17a mRNA expression in ileums of GF vs. SFB mice (n=4/gp). E. Il17a mRNA expression in gingiva of GF vs. SFB mice (n=4/gp). F. Serum ELISA analysis of IL17A levels (n=6/gp). G-H. Tnf mRNA qRT-PCR analysis; relative quantification of mRNA was performed via the 2−ΔΔCT method. G. Tnf mRNA expression in ileums of GF vs. SFB mice (n=4/gp). H. Tnf mRNA expression in gingiva of GF vs. SFB mice (n=4/gp). I. Serum ELISA analysis of TNF levels (n=5/gp). Unpaired t-test; data are presented as mean ± SEM, *p<0.05, **p<0.01, ***p<0.001.