Figure 3. SFB upregulates immune response effects in alveolar bone marrow.

A-H. Mandible bone marrow (MBM) cells were isolated and stained for flow cytometric analysis (n=6/gp); cell percentages are expressed relative to the appropriate gated population. A. Representative gating of B220⁻MHC II⁺CD11c⁺ dendritic cells (DCs). B. Frequency of B220⁻MHC II⁺CD11c⁺ DCs, gated on % B220⁻ cells. C. Representative gating of CD4+ naïve and activated T-cells. D. Frequency of CD3⁺CD8⁻CD4⁺CD62L⁺CD69⁻ naïve T-cells, gated on % CD3⁺CD4⁺ cells. E. Frequency of CD3⁺CD8⁻CD4⁺CD62L⁻CD69⁺ activated T-cells, gated on % CD3⁺CD4⁺ cells. F. Representative gating of CD3⁺CD4⁺RORγt⁺AHR⁻ T_H17 cells. G. Frequency of CD3⁺CD4⁺RORγt⁺AHR⁻ T_H17 cells, gated on % CD3⁺CD4⁺ cells. H. Representative gating of CD3⁺CD4⁺CD183⁺T-bet⁺ T_H1 cells. I. Frequency of CD3⁺CD4⁺CD183⁺T-bet⁺ T_H1 cells, gated on % CD3⁺CD4⁺ cells. J. Representative gating of CD3⁺CD4⁺CD184⁺GATA3⁺ IRF4⁻ T_H2 cells K. Frequency of CD3⁺CD4⁺CD184⁺GATA3⁺ IRF4⁻ T_H2 cells, gated on % CD3⁺CD4⁺ CD184⁺ cells. L. Representative gating of CD3⁺CD4⁺CD25⁺FoxP3⁺ T_REG cells. M. Frequency of CD3⁺CD4⁺CD25⁺FoxP3⁺ T_REG cells, gated on % CD3⁺CD4⁺ cells. Unpaired t-test; data are presented as mean ± SEM, *p<0.05, **p<0.01.