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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: Lab Invest. 2021 Dec 21;102(4):363–375. doi: 10.1038/s41374-021-00697-0

Figure 4. SFB colonization does not alter osteoblastogenesis.

Figure 4.

A-C. Immunofluorescent analysis of osteoblasts in the maxillary first molar furcal alveolar bone (n=6-7/gp). Osterix+ cuboidal cells lining bone were designated as osteoblasts (red, osterix – Rhodamine). A. Representative images of osterix+ osteoblasts lining the maxillary first molar furcal alveolar bone; arrows indicate labeled osteoblasts (200X). B. N.Ob/B.Pm = osteoblast number per bone perimeter. C. Ob.Pm/B.Pm = osteoblast perimeter per bone perimeter. D-F. Immunofluorescent analysis of osteoblasts in the secondary spongiosa of distal femur (n=5-6/gp). Osterix+ cuboidal cells lining bone were designated as osteoblasts (red, osterix – Rhodamine). D. Representative images of osterix+ osteoblasts lining the secondary spongiosa trabecular bone of distal femur; arrows indicate labeled osteoblasts (200X). E. N.Ob/B.Pm. F. Ob.Pm/B.Pm. G. Serum ELISA analysis of P1NP levels (n=8/gp). Unpaired t-test; data are reported as mean ± SEM.