Figure 1:

Schematic representation of anatomical pathways of PRV transport from bladder and colon to the medulla and the treatment protocols. Illustration of possible neural connecting pathways of the primary sensory afferent fibers from the bladder and colon to the RVM (a). Treatment protocol for naïve rats with no intravesical treatment received PRV injections into the bladder and colon on postnatal day P60 (b). Treatment protocol for female rat pups that received either intravesical protamine sulfate + zymosan or saline from P14 through P16 followed by PRV injections on P60 as described for naïve group (c). Tissues were collected from the naïve and treated groups 96 hours post viral inoculation on P64 for further analysis.