Figure 2:

Rostro-caudal distribution of bladder- and colon-linked RVM neurons. Stereotaxic representations of RVM sections (bregma −10.8 mm to 12.8 mm) taken at rostral, middle and caudal levels for analyzing PRV expression (a1,b1,c1). Representative images from the 2-color IHC show the distribution patterns of GFP+ bladder and RFP+ colon neurons in the rostral (a2-a3), middle (b2-b3) and caudal (c2- c3) RVM levels. The scale bar is 250 µm. Quantification of PRV-labeled neurons in RVM tissue sections in different RVM levels is shown in (d). Data is represented as mean ± SEM, n=3 (3 sections/level/rat). For GFP+ bladder neurons *p<0.05 middle vs rostral and caudal, for RFP+ colon neurons #p<0.05 middle vs rostral and caudal. Comparison among levels were analyzed using one-way ANOVA followed by post-hoc Bonferroni test.