Figure 6:

Immunohistochemical analysis of TpH2 expression in GFP+ RVM neurons in neonatal zymosan and saline-treated rats. Representative images show GFP-positive and TpH2-positive neurons from zymosan treated (a1-a3) and saline control (b1-b3) groups. White arrows indicate bladder specific RVM neurons co-expressing TpH2. The scale bar is 100 µm. Quantitative analysis of TpH2 positive RVM neurons in experimental and control groups, **p<0.005 vs saline controls (C). Data represented as mean ± SEM, n=3/group, 9 sections/rat. Quantitative analysis of percentage GFP+ RVM neurons expressing TpH2 in experimental and control groups, **p<0.005 vs saline controls (d). Data represented as mean ± SEM, n=3/group, 6 sections/rat. Unpaired t-test with Welch’s correction was used to compare the data between the groups.