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. Author manuscript; available in PMC: 2023 Jun 1.
Published in final edited form as: J Comp Neurol. 2021 Nov 8;530(8):1129–1147. doi: 10.1002/cne.25260

Figure 7:

Figure 7:

In situ hybridization analysis of GABA transporter VGAT mRNA expression in GFP+ RVM neurons in neonatal zymosan and saline-treated rats. RNAscope analyses of GFP and VGAT mRNA expression in zymosan (a1-a3) and saline-treated (b1-b3) groups. Bold white arrows represent the large diameter and hollow white arrows represent medium diameter GFP+ neurons expressing VGAT. The scale bar is 25µm. Quantitative data analysis for VGAT mRNA copies in large and medium diameter neurons in experimental and control groups, **p<0.005 vs saline in large diameter neurons, and **p<0.005 vs saline in medium diameter neurons (c), Data represented as mean ± SEM, n=3/group (3 sections/rat). The RVM neurons were grouped as large, 18 neurons/group and medium 27 neurons/group with average diameters of 40.8±7.7µm and 23.2±4.2µm respectively. Unpaired t-test with Welch’s correction was used to compare the data between the groups.