Figure 8:

Immunohistochemical analysis of VGAT protein expression in GFP+ RVM neurons following neonatal cystitis. Representative images of GFP and VGAT protein expression in experimental (a1-a3) and saline-treated (b1-b3) groups. The bold white arrows represent GFP+ neurons expressing VGAT, and hollow white arrows represent GFP+ neurons without VGAT expression. The scale bar is 25µm. Quantitative data of VGAT protein expression measured as integrated density of VGAT staining within GFP+ neurons for experimental and control groups, **p<0.005 vs saline controls (c). Data represented as mean ± SEM, n=3/group, 3 sections/rat). Unpaired t-test with Welch’s correction was used to compare the data between the groups.