. Author manuscript; available in PMC: 2023 Jun 1.

Published in final edited form as: J Comp Neurol. 2021 Nov 8;530(8):1129–1147. doi: 10.1002/cne.25260

Table 2-.

Probes used for RNAscope ISH protocol

RNAscope® Probe	Probes (ACDBio)	Accession No:	TSA fluorophores (Perkin Elmer) (1:500-1:1000)
GFP-C1	409011	AF2759553.1 (probe: 12-686bp)	TSA plus fluorescein NEL741E001KT
GFP-C3	409011-C3	AF2759553.1 (probe: 12-686bp)	TSA plus fluorescein NEL741E001KT
RFP-O2-C2	562541-C2	AF168419.2 9 (probe: 2-856bp)	TSA plus Cyanine 3 NEL744E001KT
Rn-Gad1-C2	316401-C2	NM_017007.1 (probe: 950-1872bp	TSA plus Cyanine 3 NEL744E001KT
Rn-Penk-C3	417431-C3	NM_017139.1 (probe: 242-1369bp)	TSA plus Cyanine 5 NEL745E001KT
Rn-Slc32a1-C1 (VGAT)	424541	NM_031782.1 (probe: 388-1666bp
Rn-Slc32a1-C3 (VGAT)	424541-C3	NM_031782.1 (probe: 388-1666bp