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. Author manuscript; available in PMC: 2022 Jun 2.
Published in final edited form as: Nat Chem Biol. 2021 Dec 2;18(4):360–367. doi: 10.1038/s41589-021-00900-9

Extended Data Fig. 1: Extended validation of glycomacrolide photoaffinity probes for target identification.

Extended Data Fig. 1:

a, Analysis of pS6 phosphorylation using FCB barcoded flow cytometry of MV-4–11 cells treated for 16 h with glycomacrolides or probes; b, Concentrations response curves in MV-4–11 cells treated for 72 h with Apop H/Apop H-PA and c, Ammo A/Ammo A-PA; d, Gel-based profiling of Apop H PA or e, Ammo A-PA adducts in MV-4–11 cells; f, Gel-based profiling of Apop A-PA targets in BaF3 cells transformed with HRas-G12V or; g Immunoblot showing specific enrichment of ATP5B in H292 cells treated with Apop A-PA; h; Gel-based analysis of diazirine and UV light dependent labeling of Apop A-PA at 1 μM.