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. 2022 Mar 30;12:5404. doi: 10.1038/s41598-022-08826-5

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Representative p53 expression in index tumors clustered by tumor grade. p53 staining, detected at variable degrees of intensity between 1 and 5% (A, E and F) and > 5% (C, G, and K) and their corresponding sections stained with (H&E) are shown for each of the GG clusters: GG 1–3 (A to D), GG 4 (E to H), and GG 5 (I to L). Images were captured at 10X magnification. Arrowhead indicates individual cells stained by the p53 antibody.